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:: THE NEW SILVER SOLUTION vs. ANTHRAX ::

IIT RESEARCH INSTITUTE

Re: IITRI Project 1708 SN1

December 18, 2001

William D. Moeller
American Biotech Labs
70 West Canyon Crest Road, Suite D
Alpine, UT 84004

Dear Mr. Moeller:

The study, IITRI 1708 SN1, investigating the sporicidal properties of 22 ppm NEW Silver Solution PLUS against anthrax is complete. The protocol and results are as follows:

Bacillus anthracis ANR-1 was grown on sporulation media, harvested into deionized water, heat-shocked, and stored as a spore suspension. The final concentration of this spore suspension was approximately 1 x 109 spores/ml.

A total of 33 conical tubes were filled with 4.95 ml 22 ppm NEW Silver Solution PLUS and inoculated with 50”l of B. anthracis spore suspension, for a final concentration of approximately 1 x 107 spores/ml. Three of the tubes were harvested immediately by dispensing 1 ml into 9 ml TSB, serially diluting in saline, and plating on TSA. Fifteen of the tubes were incubated at room temperature, while the remaining 15 were incubated at 37șC. At time points 30 min, 1 hour, 2 hours, 4 hours and 6 hours three tubes from each incubation temperature were harvested as above. An additional Time 0 control of 50”l spore suspension in 4.95 ml sterile water was also used. The plates were incubated at 37șC for 48 hours, and colonies were counted. The results in Table 1.

TABLE 1

Time
Temperature
Average CFU/ml
Log
Log Reduction
0 hour control
room temp
1.01E+07
7.01
0 hour
room temp
7.15E+06
6.65
0.15
30 min
room temp
8.88E+06
6.95
0.06
37 degrees
1.03E+07
7.01
-0.01
1 hour
room temp
8.33E+06
6.92
0.08
37 degrees
6.90E+06
6.84
0.17
2 hours
room temp
1.01E+07
7.00
0.00
37 degrees
8.97E+06
6.95
0.05
4 hours
room temp
3.23E+06
6.51
0.50
37 degrees
2.01E+06
6.30
0.70
6 hours
room temp
3.56E+06
6.55
0.45
37 degrees
8.37E+05
5.92
1.08

Minimal reduction in spore numbers was seen compared to the Time 0 control. After 4 hours, a reduction of approximately half a log (93%) was seen at both incubation temperatures, while at 6 hours a reduction of approximately 1 log (99%) was seen only at 37șC.

Environmental controls were negative for growth, and no contamination was observed on the plates.

Sincerely,


Bruce A. Gingras, Ph.D.
Study Director
Research Microbiologist
Life Sciences Operation


Margaret A. Juergensmeyer, Ph.D.
Study Supervisor
Research Microbiologist
Life Sciences Operation


Robert Sherwood, Ph.D.
Division Manager
Life Sciences Operation

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